Journal: Stem Cell Research & Therapy
Article Title: Let-7a-5p derived from parathyroid hormone (1–34)-preconditioned BMSCs exosomes delays the progression of osteoarthritis by promoting chondrocyte proliferation and migration
doi: 10.1186/s13287-025-04416-0
Figure Lengend Snippet: Let-7a-5p inhibiting the IL-6/STAT3 signaling pathway by targeting IL-6 protected chondrocytes. ( A ). Potential binding sites of IL-6 to let-7a-5p. ( B - D ). Western blot showed the protein expression of IL-6, phospho-STAT3, and STAT3 in chondrocytes. ( E - H ). PCR showed the mRNA expression of IL-6, COL-2, MMP-13 and ADAMTS5 in chondrocytes
Article Snippet: The primary antibodies to metalloproteinase-13 (MMP-13) (1: 300; Gene Tex Inc., USA) and collagen II (COL-2) (1: 200; Gene Tex Inc., USA) were used to perform immunohistochemistry.
Techniques: Binding Assay, Western Blot, Expressing